| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.242936 |
| Chromosome: | chromosome 12 |
| Location: | 1952474 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g510500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATCAGGATGCACTGCTCAGCTTGCTC |
| Internal bar code: | TTCCGCATCGGGCTATACCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 84.1871 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGCAGAGAGACTGAGAG |
| Suggested primer 2: | CATGTAGGATGTACGTGCGG |