| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243015 |
| Chromosome: | chromosome 5 |
| Location: | 3451363 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241400 | (1 of 2) 1.16.1.7 - Ferric-chelate reductase (NADH) / NADH:Fe(3+)-EDTA reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTACCGCCTGTCCCAGCTCCTGGTTCC |
| Internal bar code: | GCTTTTCATTTTCGAACCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 412 |
| LEAP-Seq percent confirming: | 99.5726 |
| LEAP-Seq n confirming: | 9551 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGCGTCACCTTGAGTC |
| Suggested primer 2: | GAGAAGCACAAAGGAGACGG |