Insertion junction: LMJ.RY0402.243057_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.243057
Chromosome:chromosome 14
Location:2746900
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g627050 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCATGTGGTTGTACGAGCGGGCTAGGAAG
Internal bar code:GTCAGTATACGTCTTTGATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTGACTTTGCCTGCTGA
Suggested primer 2:ACACACACTCCCTCTCACCC