Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.243081 |
Chromosome: | chromosome 13 |
Location: | 1999039 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATATTGGCAGTACGGCATCTTGTTTTT |
Internal bar code: | GCGCGCTGTGTTGCGGCCGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 487 |
LEAP-Seq percent confirming: | 85.5422 |
LEAP-Seq n confirming: | 71 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGTGGGCTCACGAAGTAT |
Suggested primer 2: | TATTTACGTGGGCAACAGCA |