Insertion junction: LMJ.RY0402.243093_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243093
Chromosome:chromosome 5
Location:1017426
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g246552 (1 of 2) PTHR10566//PTHR10566:SF86 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC1 PROTEIN KINASE 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGTCGCCTCTACACCACCACACGCCGG
Internal bar code:TAGCGCCAGGAGAAGGGAAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:276
LEAP-Seq percent confirming:99.5652
LEAP-Seq n confirming:458
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGTAGTGCCCAACCCGTATG
Suggested primer 2:TGGTTTCGTTTAGTTTGGGC