Insertion junction: LMJ.RY0402.243098_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.243098
Chromosome:chromosome 2
Location:3279011
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGGCCCCATTCCTAACCGCACCCTCCC
Internal bar code:TGGCTCCCTTTCACTAAGGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACTATCTCCCCCACCCC
Suggested primer 2:CCTCGGTTGTCTTGGTTTGT