Insertion junction: LMJ.RY0402.243098_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243098
Chromosome:chromosome 2
Location:3279049
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAAGTCGGAGGGTAGGGTGTTGGAGTT
Internal bar code:ACACACGGCATAGACCGGGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:172
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTACTATCTCCCCCACCCC
Suggested primer 2:CCTCGGTTGTCTTGGTTTGT