| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243105 |
| Chromosome: | chromosome 12 |
| Location: | 5357361 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529600 | RPA1 | DNA-directed RNA polymerase I subunit 1; (1 of 1) K02999 - DNA-directed RNA polymerase I subunit RPA1 (RPA1, POLR1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGACTGAGCATGCGTACTGTGTGATTT |
| Internal bar code: | TTGCAAAGGACATTGAATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 932 |
| LEAP-Seq percent confirming: | 99.7484 |
| LEAP-Seq n confirming: | 793 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAACATTGAGAAGGTGT |
| Suggested primer 2: | GTTGAGGACGAGCCTGAGAC |