Insertion junction: LMJ.RY0402.243123_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.243123
Chromosome:chromosome 6
Location:8985532
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g311650 IRK1 (1 of 1) PTHR11767//PTHR11767:SF58 - INWARD RECTIFIER POTASSIUM CHANNEL // FI16807P1; Inwardly-rectifying potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGAAGAGGCGCACCGGCAGCGAAGCATG
Internal bar code:ACGGAGAAAACGCCGGCACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:161
LEAP-Seq percent confirming:99.0196
LEAP-Seq n confirming:404
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTGGTGGTCTGGTTTCT
Suggested primer 2:TCACCACGATCTCAGCAGAC