| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243179 |
| Chromosome: | chromosome 13 |
| Location: | 3917187 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590600 | PDE32 | (1 of 1) IPR000104//IPR002073//IPR023088 - Antifreeze protein, type I // 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain // 3'5'-cyclic nucleotide phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGCACCAGCGCCGCGCCGACTTTATGC |
| Internal bar code: | TCGCTATCGGGGGGGTTCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 94.5106 |
| LEAP-Seq n confirming: | 2462 |
| LEAP-Seq n nonconfirming: | 143 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGCAAGCTCTTTTGGGT |
| Suggested primer 2: | TCACCGTGTGTGTAGGCAAT |