| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243204 |
| Chromosome: | scaffold 24 |
| Location: | 88669 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755747 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTAACCGGTGCAAGCGCACGGCCGACCG |
| Internal bar code: | CTCACGCGCGGTCTGGGGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 99.0287 |
| LEAP-Seq n confirming: | 2549 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGACTGCTGTCATGCTC |
| Suggested primer 2: | TTGTATGCCCCACAACTTCA |