| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243395 |
| Chromosome: | chromosome 8 |
| Location: | 1420883 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364300 | FAP29 | (1 of 3) PTHR10338//PTHR10338:SF110 - VON WILLEBRAND FACTOR, TYPE A DOMAIN CONTAINING // VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A; von Willebrand Factor Type A Domain Flagellar Associated Protein 29 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGGGAACACGGGCTTGCTGCTCCAGGC |
| Internal bar code: | CAGTATGGACGAGGCTCCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.6073 |
| LEAP-Seq n confirming: | 11160 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGGGAAGGAAACATGCAG |
| Suggested primer 2: | CCCATCCACTCCACTCATCT |