Insertion junction: LMJ.RY0402.243395_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243395
Chromosome:chromosome 8
Location:1420917
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g364300 FAP29 (1 of 3) PTHR10338//PTHR10338:SF110 - VON WILLEBRAND FACTOR, TYPE A DOMAIN CONTAINING // VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A; von Willebrand Factor Type A Domain Flagellar Associated Protein 29 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGAGCCGACTGGACCTGCTCACGGCGC
Internal bar code:GCCCCATCGCCAGAGCAGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:93.9024
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGCTGGCTGTGAACTGTA
Suggested primer 2:CCCATCCACTCCACTCATCT