| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243448 |
| Chromosome: | chromosome 12 |
| Location: | 3955852 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516400 | SPL24 | Pre-mRNA splicing factor; (1 of 1) PF04146 - YT521-B-like domain (YTH) | 3'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCTCGCGACGCATACCACACTTGCGT |
| Internal bar code: | CGGGATTGATAGTGCCATGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 98.6777 |
| LEAP-Seq n confirming: | 1194 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTGAATGAAGCAAAGCA |
| Suggested primer 2: | AACCTTGCGTCTTGCTTTGT |