| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243460 |
| Chromosome: | chromosome 3 |
| Location: | 2839121 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162450 | FBT4 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGCAGGCTTGCGGCGCCAGCTGTGC |
| Internal bar code: | GGCGACTTTGCAAATGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACTCGCCGTCTCGATAG |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |