| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243477 |
| Chromosome: | chromosome 10 |
| Location: | 6242554 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464750 | NAT25 | (1 of 5) PTHR23091:SF255 - ACYL-COA N-ACYLTRANSFERASES-LIKE PROTEIN; N-acetyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGCGCCCCCGCTCCAACCTTCACGAAG |
| Internal bar code: | CGTGGGACAATCGAGCGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 99.6764 |
| LEAP-Seq n confirming: | 308 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGGCACGTATGGTTTG |
| Suggested primer 2: | GTGGCTGATCTTCCATGGTT |