| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243540 |
| Chromosome: | chromosome 16 |
| Location: | 4781020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686500 | (1 of 1) PF03767 - HAD superfamily, subfamily IIIB (Acid phosphatase) (Acid_phosphat_B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCTCCCCGCACCCGTGGCGCCTCCAG |
| Internal bar code: | GATATCCGTTCCGGGTGGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 87.3171 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACACAAAGCACCCACCA |
| Suggested primer 2: | ACATCTCCTGATACAGCCCG |