Insertion junction: LMJ.RY0402.243564_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.243564
Chromosome:chromosome 9
Location:4681085
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396846 (1 of 2) PTHR22957//PTHR22957:SF148 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // RE26521P 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTAGGATCTCTCTTCTTCCTGGCAATAAA
Internal bar code:CAGGGCAGATTCCTCTGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:496
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:216
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTTACCGCGATGCAAAC
Suggested primer 2:ACCACTCAAGGCATACGTCC