Insertion junction: LMJ.RY0402.243564_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243564
Chromosome:chromosome 9
Location:4681095
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396846 (1 of 2) PTHR22957//PTHR22957:SF148 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // RE26521P 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGGTTAGCCGACGTCCATCCCTTCACG
Internal bar code:CGCTCGACATGCGGCGCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:247
LEAP-Seq percent confirming:95.6835
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTTACCGCGATGCAAAC
Suggested primer 2:ACCACTCAAGGCATACGTCC