Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.243567 |
Chromosome: | chromosome_3 |
Location: | 1324417 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g150650 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCTTACACTTGATGCTGTTTGAATTGCAGC |
Internal bar code: | GCGAATGGCCGTTAGAGGCACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 747 |
LEAP-Seq percent confirming: | 91.2644 |
LEAP-Seq n confirming: | 397 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTCAGGAGAGCAGGGAAG |
Suggested primer 2: | CGTTTGTCATGGAAATGCAC |