| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.243567 |
| Chromosome: | chromosome 3 |
| Location: | 1324417 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150650 | (1 of 2) PTHR12775:SF0 - PROTEIN RTF2 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTACACTTGATGCTGTTTGAATTGCAGC |
| Internal bar code: | GCGAATGGCCGTTAGAGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 91.2644 |
| LEAP-Seq n confirming: | 397 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCAGGAGAGCAGGGAAG |
| Suggested primer 2: | CGTTTGTCATGGAAATGCAC |