| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243619 |
| Chromosome: | chromosome 12 |
| Location: | 6000020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534961 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGCTTGGACTACGTTTATACCTGGTAT |
| Internal bar code: | AAGGCCTCCCGGTCCAAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 98.9305 |
| LEAP-Seq n confirming: | 555 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTAGAGCCACAGATCGAC |
| Suggested primer 2: | AGAGGACCCACGCTGATATG |