| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243619 |
| Chromosome: | chromosome 17 |
| Location: | 6986969 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g746247 | (1 of 1) K11430 - histone-lysine N-methyltransferase EZH2 (EZH2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACACATACTAGGACGCAGGGCACGTG |
| Internal bar code: | GGTGTCACACGCAAACATGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 99.2248 |
| LEAP-Seq n confirming: | 256 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCTCGTTTAGCTTGTTT |
| Suggested primer 2: | GTCTCTTGCCCTCTGTCTCG |