| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243652 |
| Chromosome: | chromosome 6 |
| Location: | 4268775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278249 | AST5 | Putative aspartate aminotransferase; (1 of 1) PTHR11751//PTHR11751:SF33 - SUBGROUP I AMINOTRANSFERASE RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGCGGGCCTGGACCCCATCTACATGC |
| Internal bar code: | TTTTAATGTGGCTTGATCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 99.9075 |
| LEAP-Seq n confirming: | 3239 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCGCATCCACATACATAA |
| Suggested primer 2: | TCGTATTATCGCTCACGCTG |