Insertion junction: LMJ.RY0402.243673_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243673
Chromosome:chromosome 11
Location:2489196
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g475650 (1 of 11) IPR017956 - AT hook, DNA-binding motif intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGTGGTGCGGTTTGCTCTGTGCGGTT
Internal bar code:CGCCCGATGCGCAAGGGCGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:99.7585
LEAP-Seq n confirming:413
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTAATCGGACTGCTGCTG
Suggested primer 2:CATCACGAAATGTACCGTGC