| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243685 |
| Chromosome: | chromosome 14 |
| Location: | 2002477 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621501 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTTTGAAGTGCACGTTGCCAATGTGC |
| Internal bar code: | GGTGCAAGGAACGATTGCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.1061 |
| LEAP-Seq n confirming: | 887 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTAGAGCGTCCTTGCGG |
| Suggested primer 2: | GGGCGTATCCAGATTGCTAA |