Insertion junction: LMJ.RY0402.243709_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.243709
Chromosome:chromosome 9
Location:1829514
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395950 AOX1 Alternative oxidase, mitochondrial; (1 of 2) PTHR31803//PTHR31803:SF3 - FAMILY NOT NAMED // ALTERNATIVE OXIDASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAATTACGGTAATCGTGAATGCGATCCCCA
Internal bar code:CGGGAATCTCTTTACTCCCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:950
LEAP-Seq percent confirming:99.7712
LEAP-Seq n confirming:872
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGATGGTATGTGCGAGG
Suggested primer 2:ACGCTTGAAGTCTTCGCAAT