| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243714 |
| Chromosome: | chromosome 13 |
| Location: | 1908096 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g576000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGCCTTGTTGGCAGATGTTGAAACTG |
| Internal bar code: | GGGGCACCAATCCGGCTGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 99.5728 |
| LEAP-Seq n confirming: | 2564 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTCACGGTAGCCATCT |
| Suggested primer 2: | GCTTGGTGCCTGTGACTACA |