Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.243714 |
Chromosome: | chromosome_13 |
Location: | 1908103 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g576000 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCAGGATAATGCCGCTTGTGAAGATTGCA |
Internal bar code: | ATGCAGTCTGTAGGGGGATAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 511 |
LEAP-Seq percent confirming: | 99.2296 |
LEAP-Seq n confirming: | 644 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGGTCACGGTAGCCATCT |
Suggested primer 2: | GCTTGGTGCCTGTGACTACA |