| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243734 |
| Chromosome: | chromosome 9 |
| Location: | 5659327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401256 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCCTACACCATCACAGTGCGCCCCGC |
| Internal bar code: | GGCCTTCCAACACTTGCTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.0583 |
| LEAP-Seq n confirming: | 202 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGCACACACGAGGAGTG |
| Suggested primer 2: | CAGACAGCACCTCTGCCATA |