| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243750 |
| Chromosome: | chromosome 13 |
| Location: | 5057900 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607050 | TST,THT1,TSU1,TST1 | Thiosulfate sulfurtransferase; (1 of 1) K01011 - thiosulfate/3-mercaptopyruvate sulfurtransferase (TST, MPST, sseA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTCCCAGCCGGTTGTGGGCAGCTGCGG |
| Internal bar code: | GGATGACGTGGGCTGTGCCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGACAGCCACATGTTC |
| Suggested primer 2: | ACAGCTCACACAGCATCCAG |