Insertion junction: LMJ.RY0402.243763_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.243763
Chromosome:chromosome 6
Location:2892598
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g272250 PHC27 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGACAGTGTGGGCTGACGGCGTGTTGGC
Internal bar code:GCGGGGCGGGTGTGATGTGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:393
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:376
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGCATCAAGTCGATCAGC
Suggested primer 2:GACTCTGCATGCCGTACTGA