| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243799 |
| Chromosome: | chromosome 9 |
| Location: | 5790235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402071 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGATTGTATTTGTTCGCGGGCACCAT |
| Internal bar code: | GATGACCGTCTTGGACGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 98.7772 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTCCTGTTCGTGTCACC |
| Suggested primer 2: | GCAGTACGGTTTTCCTGGAC |