| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243806 |
| Chromosome: | chromosome 16 |
| Location: | 1955731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656350 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGATGCCGCTGCAGGCATAGCCCAAG |
| Internal bar code: | CGTCGATGTGGCTAGAACTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.0678 |
| LEAP-Seq n confirming: | 1169 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGGTACATTCAAGGCGG |
| Suggested primer 2: | GCTGGGACCCGTTACTTACA |