Insertion junction: LMJ.RY0402.243821_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.243821
Chromosome:chromosome 7
Location:877631
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g318651 AOF4 (1 of 1) 1.5.3.16 - Spermine oxidase / SMO; Flavin-containing amine oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCTTTCCAACTTCTCGTGCCTGGACCC
Internal bar code:GTCCCTGACGGTGTCAGGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:103
LEAP-Seq percent confirming:16.4678
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:350
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTTACAACCCCCACCAC
Suggested primer 2:CACCCGCGGTAACTACAATC