Insertion junction: LMJ.RY0402.243845_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.243845
Chromosome:chromosome 7
Location:2203644
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGGTTCGCAGTCGGTGCCCCAGAGCT
Internal bar code:GTGGGGTGGAGCTTCTCTCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:221
LEAP-Seq percent confirming:99.3983
LEAP-Seq n confirming:2478
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAACGTCGAGCAGGTACA
Suggested primer 2:ACTTGGTTTGGTTTCGTTCG