Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.243852 |
Chromosome: | chromosome 7 |
Location: | 887279 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g318702 | (1 of 1) K11806 - WD repeat and SOF domain-containing protein 1 (WDSOF1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACCCCCCCCCTGACACTACCATGGTCAC |
Internal bar code: | CTAGGGCGAATTTGTTGGAAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 319 |
LEAP-Seq percent confirming: | 99.815 |
LEAP-Seq n confirming: | 3238 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCTCTTCCCTCATCGTA |
Suggested primer 2: | AATGCTCCACACTACCAGGG |