| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243862 |
| Chromosome: | chromosome 7 |
| Location: | 61067 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312400 | KDG1 | Diacylglycerol kinase; (1 of 2) K00901 - diacylglycerol kinase (ATP) (dgkA, DGK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCATTGGAAAAGCCATGTCTCTAGCCTG |
| Internal bar code: | TGTTTTCAGCTGTTGCGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 99.3072 |
| LEAP-Seq n confirming: | 7024 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTTCGTGACACTCCCCT |
| Suggested primer 2: | GAAACGATGTCCGTCGAAGT |