| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.243869 |
| Chromosome: | chromosome 1 |
| Location: | 5304004 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037100 | (1 of 4) PTHR24347//PTHR24347:SF138 - SERINE/THREONINE-PROTEIN KINASE // PROTEIN F32D8.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTCGAGGATGCAAAGGCGCCCCTCCCC |
| Internal bar code: | CCGGCATCGCGACGTCCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | GGGACTGAAGCTCGACTGAC |