| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.243889 |
| Chromosome: | chromosome 12 |
| Location: | 8326671 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549650 | (1 of 89) 3.6.4.13 - RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCCTGGGTGTTGGAAACGGAAAAGTG |
| Internal bar code: | CCCTTCTAAGCTGTGACGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 136 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTGTGGAGAACGGAGT |
| Suggested primer 2: | GCACGCACAAACTGAACTGT |