Insertion junction: LMJ.RY0402.243927_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.243927
Chromosome:chromosome 5
Location:3195406
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g239750 ARS13 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain; Arylsulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCGTTGTCTGAGGTGTACATGATGACTG
Internal bar code:AAGTAGTGCCTTTTTCCAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCAGCAAGTCATACTTCA
Suggested primer 2:GGAGGAGCACGTGTAACCAT