| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.243994 |
| Chromosome: | chromosome 5 |
| Location: | 1661409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243000 | (1 of 1) K14491 - two-component response regulator ARR-B family (ARR-B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCGCAGCCGCCGCAAGGAACGCACCA |
| Internal bar code: | GTCCATATTACATGTTCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 97.7343 |
| LEAP-Seq n confirming: | 3796 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTGACCAAAAAGCACG |
| Suggested primer 2: | GCCTGGAGGTGGTCTTGTAA |