| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243994 |
| Chromosome: | chromosome 5 |
| Location: | 1661410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243000 | (1 of 1) K14491 - two-component response regulator ARR-B family (ARR-B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGCGCGACGGCACTGGAGAGCACTGA |
| Internal bar code: | CGCCCCCGGGAGATGGCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 98.5965 |
| LEAP-Seq n confirming: | 2529 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTTGACCAAAAAGCACG |
| Suggested primer 2: | GCCTGGAGGTGGTCTTGTAA |