| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244047 |
| Chromosome: | chromosome 13 |
| Location: | 2407131 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579598 | SSS3B,SSS3 | (1 of 2) PTHR12526//PTHR12526:SF341 - GLYCOSYLTRANSFERASE // SUBFAMILY NOT NAMED; Soluble starch synthase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTAGTTGGTTTAGTTGGTATGGTATCTA |
| Internal bar code: | GTACGCGCGGCATGTCCCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 286 |
| LEAP-Seq percent confirming: | 99.6468 |
| LEAP-Seq n confirming: | 12978 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGGTATCCACCTGAT |
| Suggested primer 2: | ATCCCCCATCTATCTCCCAC |