| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244162 |
| Chromosome: | chromosome 4 |
| Location: | 2377928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221050 | (1 of 3) PTHR12136//PTHR12136:SF47 - STEROIDOGENIC ACUTE REGULATORY PROTEIN STAR // STEROIDOGENIC ACUTE REGULATORY-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGAGGAGGCCGGGGGAGCGAGGAGTG |
| Internal bar code: | ACGCCGGGGATTACAGGGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGGTTTGGCTGGTATGT |
| Suggested primer 2: | GACGACGAGAAGGAGGTGAG |