Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.244173 |
Chromosome: | chromosome_2 |
Location: | 4723898 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g102050 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCTTCTTGGCAGCCGCGTAGGCGTACTTG |
Internal bar code: | GCAGACTTCTACACACTGTCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 397 |
LEAP-Seq percent confirming: | 96.861 |
LEAP-Seq n confirming: | 648 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACCAATTCACAACAGTGG |
Suggested primer 2: | GACCAAGGACACGTCCAAGT |