| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244191 |
| Chromosome: | chromosome 10 |
| Location: | 1913150 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGCGAGGGAGAGAAGGGAGCCGTGGTC |
| Internal bar code: | GAGGTAAGCGCTTACGCATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 99.4987 |
| LEAP-Seq n confirming: | 5955 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCACTTACTCGACGGAGA |
| Suggested primer 2: | GTGCTGGTTAAGCAGAAGCC |