Insertion junction: LMJ.RY0402.244217_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.244217
Chromosome:chromosome 16
Location:1834895
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g655450 (1 of 1) PF14510 - ABC-transporter extracellular N-terminal (ABC_trans_N) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGTCAACACGGGCCGCACAGTGGTGTGC
Internal bar code:ACACACCCAGTTCGACAATAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGTGGTGATAAGGCACT
Suggested primer 2:GCGTCAGTCAGTGGAGTGAA