| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244221 |
| Chromosome: | chromosome 2 |
| Location: | 2437362 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091550 | WHI,EZY18,PBF2 | (1 of 1) PTHR31745:SF1 - SINGLE-STRANDED DNA-BINDING PROTEIN WHY2, MITOCHONDRIAL; Whirly domain protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAAGCTAGCGCCTCGAGTGTAAGCTACA |
| Internal bar code: | AGCGCTTGCGTATATTGTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 99.5205 |
| LEAP-Seq n confirming: | 9132 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGTGGCTCAGGATAGGA |
| Suggested primer 2: | ACATCGCTTGCTCTGATCCT |