| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.244225 |
| Chromosome: | chromosome 3 |
| Location: | 8430001 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199311 | VPS16,VCL1 | VPS16-like protein; (1 of 1) PTHR12811//PTHR12811:SF0 - VACUOLAR PROTEIN SORTING VPS16 // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCAGGCATACGGCTCGAGCAGTTGGA |
| Internal bar code: | TCTGAAGGCCGCATCGATCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1389 |
| LEAP-Seq percent confirming: | 99.6152 |
| LEAP-Seq n confirming: | 12944 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCATATACTCCTGCCGCAC |
| Suggested primer 2: | CTGAAGAGGTCTACCAGGCG |