| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.244230 |
| Chromosome: | chromosome_8 |
| Location: | 2449326 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g371650 | TATB,TATB1,TAT2 | TatB-like sec-independent protein translocon subunit | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCCCTGCGAGCCTCCGCTCCAAACCCGA |
| Internal bar code: | GGCGCCAGTTCTAGTCGCTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.6063 |
| LEAP-Seq n confirming: | 3289 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGCAAGACCTGTCAGT |
| Suggested primer 2: | GTTACCAGCTCGTCAGCCTC |